Therapeutic ultrasound design and parameters used. (A) Outline of specific ultrasound conditions used in this experiment. (B) Conditions were chosen on the basis of previous experiments that optimized gene transfer in pigs. H114H is a geometrically focused transducer with the highest intensity at 45 mm (Z = 45 mm) from the face of the transducer (Z = 0 mm), shown by a map of relative pressure measured in decibels (dB) using a hydrophone. Measurements were taken at 3 Y locations across the length of the transducer where y = −41 is the left side and y = 42 is the right side relative to the center (y = 0) of the transducer.