Ultrastructural analysis of ATG4A KD reticulocytes. (A) Transmission electron micrograph of day 18 reticulocytes from control and ATG4A KD cultures. Mitochondria are marked with arrows. Quantification of mitochondria (B), autophagic vesicles (C), and lysosomes (D). Analysis was performed on 2-dimensional sections of at least 18 reticulocytes from each group. Data shown reflect 2 hairpins per group and are plotted as mean ± SEM of a single donor. Data were analyzed using the Mann-Whitney test.