Intracellular uptake of uric acid via the urate transporter SLC2A9 in neutrophils. (A-B) Human neutrophils from healthy subjects (A, A’, and A’’) and primary human TECs (B, B’, and B’’) were stained with the anti-human SLC2A9 antibody (red) and DAPI (white, nuclei), and confocal microscopy was performed. (C-D) MFI of intracellular and surface SLC2A9 expression in human neutrophils (C) and TECs (D) compared with isotype control was determined by flow cytometry (n = 3 per group). (E) Neutrophils from healthy subjects were cultured with or without 10 mg/dL sUA for 10 and 30 minutes and the intracellular UA levels determined using an UA assay kit (n = 7-8 per group). (F) Intracellular UA levels from CXCL8-activated neutrophils in the presence or absence of 10 mg/dL sUA determined by UA assay kit (n = 6-8 per group). Data are mean plus or minus SD. *P < .05, **P < .01, ***P < .001. ns, not significant by 1-way ANOVA; DAPI, 4′,6-diamidino-2-phenylindole.