In vivo treatment reproduces the SS drug sensitivity determined in vitro. (A) Schematic representation of the treatment protocol in mice. (B) Representation of disease progression in the different groups of treatment as determined by FC analysis of PB. (C) Representative IHC analysis of the liver showing the presence of malignant SS cells specifically in vehicle-treated animals. (D) Western blot analysis of the indicated proteins in blood samples from 3 different vehicle-treated or panobinostat-treated mice. Note the increased levels of acetylated histone in the panobinostat-treated blood samples consistent with the HDAC inhibitory activity of this drug.