ATF4 induction and target genes following LASP treatment in ALL cell lines. (A) Relative expression of ATF4 protein in ALL cell lines following LASP treatment (n = 3 independent experiments; mean ± SEM). (B) Number of ChIP-seq ATF4 sites (FDR <0.05; n = 2 independent experiments per cell line and timepoint) in Nalm6 (red) and SUPB15 (blue) cells at basal conditions (Ctrl) or after 2 hours of LASP treatment (1 IU/mL; LASP). Dark red and dark blue denote strong peaks (>fivefold ChIP enrichment), whereas light red and light blue denote weaker peaks (<fivefold ChIP enrichment). (C) Genomic locations of ChIP-seq ATF4 sites are provided at basal conditions (Ctrl) and after LASP treatment (1 IU/mL; LASP) in Nalm6 and SUPB15 cells. (D) Percentage of LASP response genes (absolute log2 fold change >1.5; FDR <0.05) with (green) or without (red) promoter-associated ATF4 occupancy at basal conditions (Ctrl) and after LASP treatment (1 IU/mL; LASP). (E) Integrative genomics viewer (IGV) genome browser tracks showing ATF4 ChIP-seq and ATAC-seq of LASP response genes ASNS, SLC7A11, DDIT3, BBC3, GPRIN3, PMEPA1, CCPG1, CARD11, and TRIM2 before and after LASP treatment in Nalm6 and SUPB15 cells.