Desialylation of the MM cell surface enhances NK cell–mediated tumor cell lysis. (A-C) JJN3 and H929 MM cell lines were treated with neuraminidase (NEURA) or glycobuffer (GLYCO) and cocultured with healthy donor peripheral blood-derived NK cells that were naïve (A), IL-2 activated overnight (B), and expanded at various indicated E:T ratios (C). All cytox assays were carried out for 4 hours. Graphs represent mean specific lysis + SEM. (A-C) n = 7. Data analyzed using Student’s paired t-test. *P < .05; **P < .01; ***P < .001; ****P < .0001; ns, not significant.