Figure 3.
Higher levels of TPO in liver and plasma, but not in BM, in PF4Cre;Tgfb1flox/flox vs WT mice. (A) TPO levels in plasma were measured by ELISA (R&D Systems; P = .003; n = 6-10). (B,E) Quantitative reverse transcription PCR of TPO mRNA from liver tissue (B) and BM cells (E) from PF4Cre;Tgfb1flox/flox (PF4;Tgfb1) and WT (C57Bl/6) mice. Thrombospondin expression was calculated by the ΔCT method with a reference gene β-actin, and data are expressed as fold change over WT C57Bl/6. (C) Liver sections from PF4;Tgfb1 and WT (C57Bl/6) mice were stained with anti-TPO antibody. (D) TPO levels in BM exudates were measured by ELISA. (F) BM sections were stained with anti-TPO antibody. Nuclei were stained with 4′,6-diamidino-2-phenylindole (blue color). AU, arbitrary unit; ns, not significant.

Higher levels of TPO in liver and plasma, but not in BM, in PF4Cre;Tgfb1flox/flox vs WT mice. (A) TPO levels in plasma were measured by ELISA (R&D Systems; P = .003; n = 6-10). (B,E) Quantitative reverse transcription PCR of TPO mRNA from liver tissue (B) and BM cells (E) from PF4Cre;Tgfb1flox/flox (PF4;Tgfb1) and WT (C57Bl/6) mice. Thrombospondin expression was calculated by the ΔCT method with a reference gene β-actin, and data are expressed as fold change over WT C57Bl/6. (C) Liver sections from PF4;Tgfb1 and WT (C57Bl/6) mice were stained with anti-TPO antibody. (D) TPO levels in BM exudates were measured by ELISA. (F) BM sections were stained with anti-TPO antibody. Nuclei were stained with 4′,6-diamidino-2-phenylindole (blue color). AU, arbitrary unit; ns, not significant.

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