Macrophage characterization by multiplex immunofluorescence. (A-B) CD68⁺CD115⁺ cells after and before R². (C-D) CD68⁺CD115⁺CD172a⁺ cells after and before R². (E-F) CD68⁺CD163⁺CD172a⁺ cells after and before R². High magnification areas (40×) are shown for all cases. Intrapatient comparisons are reported. In panel A, the post-R² FL sample shows a high concentration of CD68⁺CD115⁺ cell population (CD68, yellow; CD115, pink). In panel B, the pre-R² FL sample shows a lower concentration of CD68⁺CD115⁺ cell population (CD68, yellow; CD115, pink). In panel C, the post-R² FL sample shows a high concentration of CD68⁺CD115⁺CD172a⁺ cell population (CD68, yellow; CD115, pink; CD172a/SIRPα, magenta). In panel D, the pre-R² FL sample shows a lower concentration of CD68⁺CD115⁺CD172a⁺ cell population (CD68, yellow; CD115, pink; CD172a/SIRPα, magenta). In panel E, the post-R² FL sample shows a high concentration of CD68⁺CD163⁺CD172a⁺ cell population (CD68, yellow; CD163, cyan; CD172a/SIRPα, magenta). In panel F, pre-R² FL sample shows A lower concentration of CD68⁺CD163⁺CD172a⁺ cell population (CD68, yellow; CD163, cyan; CD172a/SIRPα, magenta).