Variants in HEATR3 impair 60S ribosomal subunit accumulation. (A) Representative polysome profiles of lysates from LCLs derived from healthy individuals or patients (P2 and P4). The 40S subunit, 60S subunit, and 80S monosome are labeled. The vertical arrow highlights the reduction of 60S subunits in the samples from P2 and P4. (B) Representative polysome profiles of lysates from LCLs transduced with lentiviruses expressing HEATR3 or GFP complementary DNA. At least 3 independent experiments were performed with each cell line (panels A and B).