Platelet ITAM receptor signaling is critical for VT but not AT. (A) WT or Clec2mKO mice were treated with JAQ1 antibody to deplete GPVI from circulating platelets. Thrombus weight was determined after 48 hours of IVC flow restriction. (B) Integrin activation response (JON/A-PE binding) after convulxin (Cvx) stimulation of platelets isolated from untreated or JAQ1-treated WT mice. (C) Thrombus weights after 48 hours of IVC stenosis in untreated (control) or JAQ1-treated WT mice. (D) JON/A-PE binding following Cvx stimulation of platelets isolated from dimethyl sulfoxide (vehicle)-treated or ibrutinib-treated WT mice. (E) Thrombus weights after 48 hours of IVC stenosis in vehicle- or ibrutinib-treated WT mice. (F) Vessel occlusion times after FeCl₃ treatment for 3 minutes in vehicle- or ibrutinib-treated mice. Dots represent individual mice, bars indicate medians. *P < .05; **P < .01; ****P < .0001.