MiR-31 is dispensable in T cell–mediated GVL response. (A-E) WT or miR-31 KO T cells (2.75 × 10⁶; CD45.2⁺) plus WT T cell–depleted BM (CD45.1⁺) were transferred into lethally irradiated B6D2F1 mice. On the day of BMT, 5000 luciferase-transduced P815 cells were IV injected into these recipients. Clinical scores (A), macroscopic photos of recipients on day 40 (B), and survival (C) are shown. P815 growth was monitored by bioluminescence imaging (D). Frequencies of IFN-γ⁺, tumor necrosis factor α–positive (TNFα⁺), granzyme B⁺, perforin⁺, and CD107a⁺ cells in gated live H2K^d-CD45.2⁺CD8⁺ cells (E) are shown in recipient spleens on day 50 post-BMT. Data shown are from 1 representative of 2 individual experiments (n = 4-5 mice per group for each experiment). **P < .01.