LAMA4 is expressed in vascular and mesenchymal cells of adult mouse BM. Representative confocal image of immunofluorescence staining of LAMA4, SCA1, and endomucin (EMCN) in BM of adult mice at steady-state. (A,B) Colocalization of LAMA4 and SCA1(A) or EMCN (B) in BM of adult Lama4^+/+ mouse femur. (C) LAMA4 staining in Lama4^−/− mouse tibia shows specificity of LAMA4 staining. (D) Representative FACS profile for sorting mesenchymal stem cells (MSCs), mesenchymal progenitors (MPC), endothelial cells (ECs), and mature stromal cells (CD44⁺ and CD44⁻SCA1⁻CD51⁻) from adult Lama4^+/+ and Lama4^−/− mouse BM. (E) Q-PCR analysis of Lama4 mRNA expression in the Lama4^+/+ and Lama4^−/− stromal cells and CD45⁺TER119⁺ hematopoietic cells. Each dot represents the average values of triplicate measurements. The horizontal bars indicate the mean of the expression levels. The data are from 2 to 4 independent sorting experiments. The P values in the figure were determined by unpaired Student t test (MPCs vs ECs) or Mann-Whitney test (MSCs vs ECs).