Metabolomic analyses of primitive erythroid precursors at E10.5 and E12.5 of gestation. (A) Erythroid precursors were isolated from the bloodstream of murine embryos at E10.5 and E12.5 and analyzed by LC-MS metabolomics. (B-D) Significant changes between the 2 groups (n = 5) were observed by unsupervised PCA analysis (B), partial least square discriminant analysis (variable importance in projection [VIP] plots for PC1 are shown) (C), and hierarchical clustering analysis (HCA) of the top 75 significantly different proteins (t test) (D). Analyses in panels B to D were performed upon normalization against a pool of data obtained from E10.5 samples and autoscaled. (E) Volcano plot representation of original raw data.