Lung inflammation induced by zymosan challenge or remote tissue inflammation in mice with global or LysMCre-mediated deletion of NOX2. (A) Schema for zymosan-induced lung inflammation in WT or Ncf2^fl/fl and Cybb^KO (NOX2^KO) or Ncf2^LysMcre mice. (B) BAL leukocyte counts and total polymorphonuclear leukocytes (PMNs), as identified by microscopy in 3 mL of BAL from WT and Cybb^KO mice of different ages after intranasal administration of zymosan (n ≥ 4 mice in each group). (C) BAL leukocyte counts and total PMNs as identified by microscopy in 3 mL of BAL from Ncf2^fl/fl and Ncf2^LysMcre mice with different ages following intranasal administration of zymosan (n ≥ 5 mice in each group). (D) Schema for analysis of lung inflammation after intraperitoneal administration of zymosan in WT and Cybb^KO mice. (E) BAL leukocytes, and total PMNs in 3 mL of BAL from the indicated groups of WT and Cybb^KO mice of different ages after intraperitoneal zymosan (n ≥ 4 mice in each group). (F) BAL CXCL2 levels in samples from the indicated groups after intraperitoneal zymosan (n ≥ 4 mice in each group). (G) Hematoxylin-eosin staining of lung tissues from WT and Cybb^KO mice after intraperitoneal zymosan. Representative image from 1 of 4 samples in each group. Arrowheads, focal neutrophil infiltrates. Scale bar, 250 μm. (H) Representative images of immunohistochemistry for MPO to identify lung PMNs after intraperitoneal zymosan. Arrows, MPO⁺cells. Representative image from 4 samples of each group. Scale bars, 250 μm. (I) Schema for analysis of lung inflammation after intraperitoneal zymosan in 12-week-old Ncf2^fl/fl and Ncf2^LysMCre mice. (J) BAL leukocyte counts, percentage of PMNs by microscopy, and total PMNs in 3 mL of BAL from naive (data also shown in Figure 2A) or zymosan-challenged Ncf2^fl/fl and Ncf2^LysMCre mice. Bar graph data are expressed as the mean ± standard error of the mean. Experiments were repeated at least twice. The Student t test was performed to compare results between each genotype for each age group in panel B. *P < .05; **P < .01. One-way analysis of variance was performed followed by Tukey’s post hoc analysis for data (E-F,J). *P < .05; **P < .01; ***P < .001; ****P < .0001.