AMs from mice with global or LysMCre-mediated deletion of NOX2 have increased responses to TLR stimulation. (A-B) AMs isolated from BAL of Ncf2^fl/fl and Ncf2^KO mice were stimulated with Pam3CSK4 or LPS, and gene expression was assayed by qRT-PCR in 4-week-old (A) and 12-week-old (B) mice. (C) AMs isolated from BAL of 12-week-old Ncf2^fl/fl and Ncf2^LysMCre mice were stimulated with Pam3CSK4 or LPS, and gene expression was assayed by qRT-PCR. Bar graph data are expressed as the mean ± standard error of the mean. Experiments were repeated at least twice. The Student t test was performed for samples distributed between 2 groups. *P < .05; **P < .01; ***P < .001; ****P < .0001.