The role of LDHA in MK differentiation is independent of lactate. (A) Relative content of lactate in plasma (n = 9) and circulating platelet (n = 3). (B) Extracellular acidification rate (ECAR) in LdhA^f/f and LdhA^−/− mice platelets (n = 3). (C) Statistical analysis on the ratio of PPF-MKs/total MKs in LdhA^f/f and LdhA^−/− mice fetal liver MKs with different lactate concentrations (n = 3). (D) Oxygen consumption rate (OCR) in LdhA^f/f and LdhA^−/− mice platelets (n = 3). (E-F) Total reactive oxygen species (ROS) of LdhA^f/f and LdhA^−/− mice platelets and bone marrow MKs were detected by 2′,7′-dichlorofluorescin diacetate (DCFH-DA) probe in resting and Rosup-stimulated (50 μg/mL) group (n = 4). ROS-inducing reagent Rosup (Beyotime) was used as a positive control for ROS level. (G-H) Mitochondrial ROS of LdhA^f/f and LdhA^−/− mice platelets (n = 5) and bone marrow MKs (n = 4) were detected by MitoSOX Red (Thermo Fisher Scientific). *P < .05, ****P < .0001. MFI, mean fluorescence intensity; ns, not significant.