Hepatic expression of p.(Cys1084Tyr) abolishes VWF secretion in VWF^−/− mice. (A) Following hydrodynamic gene transfer of the cysteine variant mouse Vwf cDNA, no p.(Cys1084Tyr) VWF was detectable in the plasma of VWF^−/− mice. Data represent mean ± SEM, ***P < .001. (B) DAB peroxidase staining of formalin-fixed paraffin-embedded mouse liver sections showed equivalent intracellular expression of both WT-VWF and p.(Cys1084Tyr) VWF in ∼5% of hepatocytes.