Impact of Grin1 deletion on platelet phenotype. (A-B) Scatterplots of platelet counts (A) and tail bleeding time (B) in WT, Pf4-Grin1^+/−, and Pf4-Grin1^−/− mice (8-17 biologic replicates per group). (C) Line graphs showing median fluorescence intensity of the platelet surface activation markers CD62P and JON/A after activation with ADP (1, 2.5, 5, and 10 μM) (i), thrombin (0.01, 0.25, and 0.5 U/mL) (ii), and convulxin (0.05 and 0.5 μg/mL) (iii), examined by flow cytometry. Four independent experiments were performed. (D) Scatterplots showing platelet aggregation as a percentage of area under the curve. Platelets were stimulated with either ADP (2.5, 5, and 10 μM) or thrombin (0.025, 0.05, and 0.1 U/mL), and platelet aggregation was measured by light transmission aggregometry. Three to 5 independent biologic experiments were performed, with 3 technical replicates per group. Data points are mean ± SEM. Statistical significance is shown (2-tailed Student t test for panels A and B [with Welch correction]; 2-way analysis of variance for panels C and D).