Comparison of VAF determined by sequencing using the 3 techniques: whole blood NGS, CD34⁺ cell culture NGS, and single-cell sequencing. (A) Plot showing VAFs of the indicated mutants in whole-blood (red circle), single-cell (blue square), or CD34⁺-cultured cells without treatment (green triangle). (B) Correlation between single-cell sequencing and whole blood NGS on 37 variants described in supplemental Table 1. VAF, variant allelic fraction.