Transcriptomic signature of LP and morphea cGVHD skin lesions. (A) Venn diagram showing the overlap between DEGs in lesional skin of cGVHD as compared with healthy controls (CONT). LP vs CONT in blue, morphea vs CONT in green, and LP vs morphea in red. (B-C) Volcano plot with log2FC and −log10 P values for the DEGs between LP (n = 8) (B) and morphea (n = 5) (C) cGVHD skin lesions vs CONT (n = 6). The most upregulated genes are toward the right, the most downregulated genes are toward the left, and the most statistically significant genes are toward the top. Genes of interest are encircled. (D) Scatterplot of the expression FCs of DEGs in lesional cGVHD. (E) Heatmap of the 50 most upregulated DEGs shared between LP and morphea, defined by contrasting lesional skin of cGVHD and CONT. Levels of expression for most of the DEGs differed in the 2 pathologies. Data are presented as log2 transcripts per million (TPM) expression value. Expression values are depicted according to the color scale. TPM values were estimated for each experimental dataset. A pseudo-count of 0.001 was added to each transcript before log2 transformation. Annotations are colored based on the TPM property, ranging from blue for 0, through to white for the mean TPM, up to red for the highest TPM for any gene in the sample.