Delayed caADAMTS13 administration after tMCAo and I/R injury in mice reduces IL-1α expression by peri-infarct microglia. (A-B) Microglia in the brains of vehicle- and caADAMTS13-treated animals, respectively, were stained by IF for both Iba1 and IL-1α. Images are representative fields of view from the peri-infarct region of the ipsilateral hemisphere and an equivalent region of the contralateral hemisphere. Each field of view is shown at 2 magnifications. Scale bars, 100 or 20 μm. (C) These images, and images taken from the striatum and cortex of both hemispheres, were analyzed by automated particle counting to identify activated microglia, based on morphology in Iba1 staining, or IL-1α–expressing microglia. Error bars represent mean ± standard deviation. Single comparisons were performed using an unpaired Student t test. *P < .05, **P < .01, ***P < .001, ****P < .0001.