caADAMTS13-mediated dissolution of preformed platelet aggregates in the mouse brain following tMCAo and I/R injury. (A-B) Visualization of platelet aggregates (white arrows) in vehicle and caADAMTS13-treated animals, respectively, was achieved by IF staining for the constitutive platelet marker CD41. Images are representative fields of view from either the striatum or cortex of the ipsilateral hemisphere and equivalent regions of the contralateral hemisphere. Each field of view is shown at 2 magnifications. Scale bars, 100 or 50 μm. (C) Thresholded single channel images were used for automatic particle counting in ImageJ, selecting aggregates based on particle size (0-10, 10-30, and >30 μm²). Error bars represent mean plus or minus standard deviation. Single comparisons were performed using an unpaired Student t test. *P < .05, **P < .01.