Platelet-specific deletion of SHARPIN reduces neutrophil recruitment in a peritonitis model of sterile inflammation. (A) SHARPIN^fl/fl GPIbα-Cre⁻ and SHARPIN^fl/fl GPIbα-Cre⁺ mice were injected with thioglycollate medium or saline and euthanized at 4 or 24 hours, and peritoneal lavage fluid was harvested. The number of neutrophils was determined as described in Methods (n = 5; **P < .01). (B) Western blot of lysate from SHARPIN^fl/GPIbα-Cre⁻ or SHARPIN^fl/fl GPIbα-Cre⁺ mouse platelets or from harvested peritoneal lavage fluid, subjected to SDS-PAGE and blotted for SHARPIN. β-actin re-probes were used as a loading control. The blots are representative of results obtained with lysate from 3 mice each.