ANGPTL2 derived from other niche cells or HSCs has no effect on HSC activities. (A-B) The repopulation ability of donor cells isolated from primary Angptl2^fl/fl or Prx1-Cre; Angptl2^fl/fl recipient mice was determined by flow cytometric analyses (A) at the indicated time points after transplantation (B; n = 5). (C) The multilineage contribution of donor cells in peripheral blood was quantified at 16 weeks after transplantation (n = 5). (D-E) The repopulation ability of donor cells isolated from primary Angptl2^fl/fl or Pf4-Cre; Angptl2^fl/fl recipient mice was determined by flow cytometric analyses (D) at the indicated time points after transplantation (E; n = 5). (F) The multilineage contribution of donor cells in peripheral blood was quantified at 16 weeks after transplantation (n = 5).