CSF and cytokine analysis of pre/post infusion samples. (A) Detection of tisagenlecleucel by RNA (Nanostring) demonstrating higher levels of the WPRE element in patients who achieved a CR. (B) Detection of tisagenlecleucel in the CSF of patient 008 (21 CAR+ cells/μL CSF) on day +7 by flow cytometry. (C) Pathway analysis and differential gene expression of patients who achieved a CR as best response vs others. Increase over non-CRs shown. (D) Representative serum and CSF cytokine analysis showing fold change from preinfusion baseline vs day +7. Grade 3 ICANS event shown in red.