Mitochondrial activity was ablated by panobinostat. (A) Volcano plot showing differentially expressed genes in the Nalm6 cell line treated with 10 nM panobinostat vs control for 24 hours (P < .05, Log fold change >1). (B-C) Enrichment score plots from GSEA using RNA-seq data from Nalm6 treated with 10 nM panobinostat vs control. (E) Relative level of mitochondrial related genes expression in Nalm6 treated with 10 nM panobinostat vs control for 24 hours. (F) Quantitative of ROS after staining with DCFH-DA in Nalm6 treated with 10 nM panobinostat vs CON for 24 hours. MFI, mean fluorescence intensity; NES, normalized enrichment score. Data represent mean ± SD (n = 3). Data with statistical significance are as indicated: *P < .05, **P < .01, ***P < .005, ****P < .001.