Deletion of PKM2 in myeloid cells improves stroke outcome in the filament and embolic models in a preexisting comorbid condition of hyperlipidemia. (A) Schematic of experimental design. (B-E) Filament model; n = 10-11 male mice. (B) Left: representative magnetic resonance imaging from 1 mouse of each genotype on day 1. White is the infarct area. Right: corrected mean infarct area of each genotype. (C) Survival rate between day 0 and day 7 after 60 minutes of transient ischemia. (D) mNSS in the same mice at days 1, 3, and 7 based on spontaneous activity, symmetry in the movement of 4 limbs, forepaw outstretching, climbing, body proprioception, and responses to vibrissae touch (higher score indicates a better outcome). (E) Fall latency in the accelerated rota-rod test. (F-I) Embolic model; n = 10 male mice. (F) Infarction (%), (G) survival rate, (H) mNSS, and (I) fall latency. (F) Left: representative magnetic resonance imaging from 1 mouse of each genotype on day 1. White is the infarct area. Right: corrected mean infarct area of each genotype. The animals that successfully completed the particular neurologic test were included in the analysis (see exclusion/inclusion criteria in “Methods”). Data are from an unpaired Student t test, mean ± SEM (B-F) or median ± range (D-E,H-I). Comparison of survival curves was evaluated by log-rank (Mantel-Cox) test (C,G) or by repeated measures ANOVA (Kruskal-Wallis test) followed by Fisher’s LSD test (D-E,H-I).