FLI1 is a candidate regulator for VWF based on differential expression and siRNA knockdown. (A-C) scRNA-seq data demonstrated decreases in FLI1 expression in 3 expression analyses, subject level-expression analysis (A), all cells-expression analysis (B), and control EC-expression analysis (C). (D) FLI1 shows decreased expression via quantitative polymerase chain reaction analysis in HUVECs after FLI1 siRNA knockdown (light green) compared with negative control siRNA knockdown (red). (E) VWF shows decreased expression via quantitative polymerase chain reaction analysis in HUVECs after FLI1 siRNA knockdown (light green) compared with negative control siRNA knockdown (red). VWF siRNA knockdown is shown in purple. (F) VWF promoter reporter assay expressing fluorescent eGFP in lentiviral-transduced HUVECs. After siRNA transfection with either negative control siRNA knockdown (red) or FLI1 siRNA knockdown (light green), there is decreased reporter activity in FLI1 siRNA compared with negative control siRNA. For all experiments, N > 3, and P values of significant relationships are shown as analyzed by Wilcoxon test or Kruskal-Wallis test with Dunn’s multiple comparison test. Error bars represent means ± standard error of the mean. Statistical significance is shown with asterisks compared with control siRNA (***P < .001, ****P < .0001).