Cluster-based differential gene expression of VWF and CDH5. (A) TSNE plot demonstrates 8 primary clusters of endothelial cells as determined by their overall transcriptional pattern, regardless of initial control EC vs low VWF ECFC phenotype. (B-C) Each cluster identified in panel A is then analyzed for VWF expression and CDH5 expression as shown in panels B and C, respectively, with the top row of each gene in panels B and C corresponding to clusters 1 to 4, and the bottom row corresponding to rows 5 to 8. (D) Finally, the relative distribution (% of total) of control ECs vs low VWF ECFCs are shown for each individual cluster.