Evidence of transcriptional downregulation and left-shifting of VWF expression. (A) Average VWF expression demonstrates a significant decrease in VWF mRNA copy number in low VWF ECFCs compared with control ECs. Average VWF expression for all cells was 7 UMI/cell. (B) Waffle plot of all sequenced cells (low VWF ECFCs and control ECs) demonstrating the overall distribution of high (>7 UMIs of VWF/cell) VWF expression, low VWF Expression (≤7 UMIs of VWF/cell), and no VWF expression (0 UMIs of VWF/cell). (C-D) Normalized histogram plots of VWF expression (UMI) for control EC (C) and low VWF ECFCs (D) cohorts. A mixed modeling algorithm determines the number of gaussian populations that may make up the overall distribution of the histogram. Four subpopulations (identified by the pink, teal, purple, and green curves), each with their own gaussian distribution, are predicted to create the overall population distribution in both control ECs and low VWF ECFCs. Statistical significance analyzed with Mann-Whitney U test; significance is shown with asterisks (****P < .0001).