Mutational landscape of 50 cases of DLBCL-AE. Bar graph shows mutated genes in 4 or more cases. The results are given in number of mutated cases per gene. The colored bars indicate the presence (orange) or absence (blue) of IRF4 rearrangement. A diagram of the relative positions of driver mutations is shown for IRF4, CARD11, CD79B, and MYD88. Multiple passenger mutations for IRF4 also are depicted. The x-axis indicates amino acid positions. The approximate location of somatic mutations identified in each gene is indicated. IRF4 mutations are mainly in the DNA binding domain. All CD79B mutations are located in a hotspot Y197 in the immunoreceptor tyrosine-based activation motif (ITAM). Domains of the protein are represented according to the Uniprot database (www.uniprot.org).