Oligoclonality in TCLs. (A) Schematic presentation of the TCR gene rearrangement during T-cell development. The arrows represent rearrangement on each chromosome mapped to different stages of a normal human thymocyte. The thin arrow for TCRβ symbolizes the 60-40 rule. Predicted time points of the transformation in various subtypes of TCLs are shown at the bottom. (B-C) WES and WGS samples (n = 367) were analyzed to identify the TCRα^DNA, TCRβ^DNA, and TCRγ^DNA clonotypes. (C) The frequency of the most abundant TCRβ^DNA clonotype was plotted vs the added frequency of the 2 most frequent TCRγ^DNA clonotypes or the 2 most abundant TCRα^DNA clonotypes for each sample. (D) A similar correlation plot of the frequency of dominant TCRβ^RNA clonotypes vs the dominant TCRα^RNA clonotypes (data from 196 WTS samples). DP, double positive; ISP, immature single positive; SP, single positive.