JAM-C signal is enhanced in the vicinity of BV signals but CD138 signal is diminished in these areas. Representative confocal microscopy image of intact long bones of (A) an MM-bearing mouse in comparison with (B) a nonaffected BM compartment (n = 3 mice); channel signals for BVs are shown in red (CD144-AF647), for CD138 are shown in green (CD138-AF488), and for JAM-C are shown in blue (JAM-C-AF555). Arrows indicate MM cells in proximity with BV signals that display higher JAM-C signal and lower CD138 expression. (C) Graphical analysis of mean fluorescence signal intensity of CD144 comparing CD138^high and CD138^low/neg JAM-C⁺ cells in MM-harboring BM regions. Unpaired, two-tailed Student t tests were used. Error bars indicate SD.