KDM5A inhibition induced NB4 differentiation. (A) Differentiation markers CD11b and CD11c in NB4 cells treated with indicated concentrations of a KDM5 inhibitor, CPI-455, measured by using flow cytometry. Statistical significance was determined by using Student’s t test. ***P < .001, ****P < .0001, P values for percentage of CD11b⁺ cells. #P < .05, ##P < .01, P values for percentage of CD11c⁺ cells. (B) The morphology of NB4 cells treated with dimethyl sulfoxide (DMSO) or 20 μM KDM5 inhibitor CPI-455. Bar = 15 μm. FACS, fluorescence-activated cell sorting.