KDM5A KO led to the increase of H3K4me2 at promoters and 5' intragenic regions correlated with upregulation of gene expression. (A) Heatmap analysis of the ChIP-seq profiles of KDM5A in NB4 and H3K4me2 and H3K4me3 in the control (Ctrl) and KDM5A-KO NB4 cells. Gene bodies with upregulated H3K4me2 (in KDM5A-KO NB4 cells) ± 3 kb are shown, sorted according to H3K4me2 binding intensity in control NB4. (B) ChIP-seq signal density plots showing the global levels of H3K4me3 and H3K4me2 at all genes ± 3 kb. (C) Box plot showing the expression levels of genes with increased H3K4me2 in KDM5A-KO, in the control and KDM5A-KO NB4 cells. Gene expression levels are presented as log₂CPM. Statistical significance was determined by using Student’s t test. ****P < .0001.