Further refinement of CombiFlow by including more PM markers. (A) Fish plots depicting mutational changes between diagnosis and relapse for Patient 20. (B) tSNE landscapes of Patient 20 created by the 5 markers (top) or aberrant markers selected from the 36-marker panel (bottom). (C) PCAs depicting 40 clusters colored by condition: diagnosis (red circle), relapse (blue square), and healthy (green triangle). PCAs were based on the 5 markers (left) or aberrant markers selected from the 36-marker panel (right). (D) Bar graphs depicting the ranking of the included markers for PC1 and PC2 based on the 5 markers (left) or aberrant markers selected from the extended 36-marker panel (right). (E) Fish plots depicting mutational changes between diagnosis and relapse for Patient 21. (F) tSNE landscapes of Patient 21 created by the 5 markers (top) or aberrant markers selected from the 36-marker panel (bottom). (G) PCAs depicting 40 clusters colored by condition: diagnosis (red circle), relapse (blue square), and healthy (green triangle). PCAs were based on the 5 markers (left) or aberrant markers selected from the 36-marker panel (right). (H) Bar graphs depicting the ranking of the included markers for PC1 and PC2 based on the 5 markers (left) or aberrant markers selected from the extended 36-marker panel (right).