Analysis of disease progression according to longitudinal CombiFlow analysis. (A) Gating of the main compartments according to CD45 expression and side scatter area (SSC-A) for the merged FCS files from Patient 3. CD45^– cells are gated out. (B) tSNE landscape colored by condition for all included samples (left) or per sample (right). (C) Expression of included markers per condition with the MRD-specific clusters separated to identify the subpopulation most likely to have caused relapse. (D) Expression of CD13 and CD97 on the tSNE landscape per sample. FSC-A, forward scatter area.