Laser-induced injury in WT vs FV Leiden mice. (A) Time lapse images from representative WT (top row) and FV^Q/Q mice (bottom row) after laser injury in cremaster arterioles. Images are binary representations of 2D confocal fluorescence images overlaid on the brightfield. Platelets are shown in red, P-selectin⁺ area in green, and fibrin area in blue (overlay colors are shown in the color wheel). White arrow indicates the direction of blood flow. Scale bar in each image, 10 μm. (B-D) Graphs show CD41⁺, P-selectin⁺, and fibrin⁺ areas over time after laser injury of cremaster arterioles in WT (red) and FV^Q/Q (blue) mice. Values are mean ± standard error of the mean (SEM). (E-G) Graphs show area under the curve (AUC; mean ± SEM) for platelets and P-selectin and fibrin areas over time. Statistics were calculated using the Mann-Whitney test; n = 49 injuries from 4 WT mice and 46 injuries from 4 FV^Q/Q mice.