CXCR5 expression level on fCD8s impacts their migration to the GCs. (A) Number of migrated cells in response to CXCL13 per 1 million LNMCs (n = 4 per group). Graph shows mean and standard error of the mean. Cells that migrated in the no CXCL13 condition are shown in gray. Data are combined from 4 experiments done in duplicate wells for each sample. A Student t test analysis was performed on migrated cell numbers after subtracting the background. (B) Positive correlation between CXCR5 expression on cells and their relative cell migration in response to CXCL13 (n = 4 per group). (C) Representative images and graph showing that the majority of CD8⁺ T cells (red) are excluded from the GC (green) that harbors HIV-infected cells, as depicted by p24 staining (orange) (n = 4 per group). The images were acquired at 40× maginification.