Results of liquid biopsy of vitreous body fluid from 8 cases of VRL. Allelic frequencies of mutations analyzed by targeted NGS of cell-free DNA isolated from vitreous body fluids (blue) are represented in comparison with allelic frequencies obtained from the cellular samples (red; Figure 1). In 6 cases MYD88 hotspot codon 265 was analyzed using single amplicon sequencing (left). In cases 19 and 15, mutations in CARD11 and/or PRDM1 were analyzed by using panel sequencing (case 19: CARD11 c.1871A>C/p.Q624P; case 15: CARD11 c.1078A>G/p.M360V, c.1034A>G/p.E345G, both with similar allelic frequencies, PRDM1: c.1801C>T/p.R601W) (right).