LC vaccines after ASCT stimulate antigen-specific CD4 and CD8 T-cell cytokine secretion and activation epitope expression. Postvaccination recall responses to CT7, MAGE-A3, and WT1 mRNA-electroporated LCs were measured as the fold change increase above prevaccine baseline using PBMCs at 1 and 3 months after vaccines (A-B) and BMMCs at 3 months after vaccines (C-D). Fold change in CD4 (A,C) and CD8 (B,D) T-cell proinflammatory cytokine (IFN-γ, IL-2, and TNF-α) secretion and activation epitope (CD107a and Mip-1β) expression. Pooled data (mean ± standard deviation) are shown for patients from the control (red circles) and vaccine (blue squares) arms. ns, not significant.