GATA2 suppression of IRF8 levels restricts common dendritic cell progenitor production. (A) Volcano plot of differentially expressed genes mined from transcriptome analysis of lineage-depleted wild-type and −77^−/− fetal livers cultured for 3 days as previously described.²¹  GEO accession: GSE133606. DC genes included factors involved in DC differentiation and/or differentially expressed in DC progenitor populations.³⁸ Gata2 downregulation, resulting from the −77 enhancer deletion, is also depicted. (B) Representative flow cytometry analysis of monocyte-dendritic cell progenitor (MDP) and common dendritic cell progenitor (CDP) populations. Quantitation of the frequency of CDPs and MDPs within the Flt3⁺M-CSFR⁺Ly6C⁻ CMP pools (C) and of total cells per liver (D) from 7 litters (−77^+/+Irf8^+/+, n = 7; −77^−/−Irf8^+/+, n = 5; −77^−/−Irf8^+/−, n = 8; −77^−/−Irf8^−/−, n = 7; −77^+/+Irf8^−/−, n = 3). Error bars represent mean ± SEM. *P < .05, **P < .01, ***P < .001; Welch’s unequal variance t tests. Relevant statistical comparisons for panel C are described in "Results."