Figure 2.
Identification of MAZ by mass spectrometry. (A) Schematic representation of the affinity purification screen. (B and C) Western blots (left) and EMSA (right) showing that the intensity of species (d) is dependent on MAZ expression using knockdown (siRNA) (B) or overexpression (pCD-SAF1) (C) experiments. (D) MAZ is recruited to the active α-globin promoter in vivo. Analysis of MAZ binding at the α-globin locus in EBV lymphoblasts, K562 cells, and human primary erythroid cells by ChIP-qPCR. The y-axis represents enrichment over the input DNA, normalized to a control sequence in the human 18S gene. The x-axis indicates the TaqMan probes used. The position of probes within the α-globin cluster are indicated on the heading map. The α-globin genes themselves are covered by 3 probes (Pr/Ex1, Ex2, and Ex3). Error bars correspond to 1 standard error of the mean from 2 independent ChIPs.

Identification of MAZ by mass spectrometry. (A) Schematic representation of the affinity purification screen. (B and C) Western blots (left) and EMSA (right) showing that the intensity of species (d) is dependent on MAZ expression using knockdown (siRNA) (B) or overexpression (pCD-SAF1) (C) experiments. (D) MAZ is recruited to the active α-globin promoter in vivo. Analysis of MAZ binding at the α-globin locus in EBV lymphoblasts, K562 cells, and human primary erythroid cells by ChIP-qPCR. The y-axis represents enrichment over the input DNA, normalized to a control sequence in the human 18S gene. The x-axis indicates the TaqMan probes used. The position of probes within the α-globin cluster are indicated on the heading map. The α-globin genes themselves are covered by 3 probes (Pr/Ex1, Ex2, and Ex3). Error bars correspond to 1 standard error of the mean from 2 independent ChIPs.

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