Sex-specific differences in SCD-mediated reduction of trabecular bone in distal epiphysis of mice at 3 and 5 months. (A) Representative microCT image of an entire femoral bone, including a microCT scan of the distal femur with the distal epiphysis outlined in red. (B) Representative three-dimensional heat maps of trabecular morphology in the epiphyseal region of the distal femur in AA, AS, and SS in 3-month-old (left) and 5-month-old (right) male and female mice. A pseudocolor scale of blue (0 mm) to red (0.09 mm) depicts trabecular thickness. Trabecular bone parameters generated by microCT scans include: trabecular thickness (C); BMD (D); trabecular spacing (E); and connective density (F). AA = black, AS = gray, SS = red. Data are expressed as mean ± standard deviation. Statistical significance, *P < .05, determined by two-way analysis of variance with Tukey’s post hoc test; n = 4 to 6 mice per group; scale bar, 100 μm. Bone Min. Den., BMD; HA, hydroxyapatite.