Bone mechanical testing parameters generated by 4-point bending technique in 3-month-old AA, AS, and SS mice. *P < .05, determined by two-way ANOVA with Tukey’s post hoc test, n = 4-6 mice per group, scale bar 100 μm. (A) Schematic diagram of 4-point bending mechanical testing on femoral cortical bone. Based on displacement and force measurement graph and geometry obtained from microCT imaging, stiffness (B) and ultimate stress (D) were calculated, and maximum force (C) were compared (n = 6-8 mice per group).