In vitro aggregation and ex vivo thrombus formation of platelets from NS patients. (A) Platelets from healthy controls and NS patients were stimulated with different doses of collagen. Representative aggregation curves are shown. Graph represents the percentage of maximal aggregation (mean ± SEM; n ≥ 5 healthy controls and n ≥ 6 NS patients [patients 1 to 10; Table 2]). (B) Anticoagulated whole blood from healthy controls or NS patients, where platelets were labeled with DiOC₆, was perfused through Cellix Vena8 Fluoro+ biochips at arterial physiological shear rate of 500 s⁻¹ on fibrillar collagen matrix over 3 minutes. Thrombus formation was visualized in real time by videomicroscopy. Profiles shown are representative of 4 healthy controls and 4 NS patients (patients 5 to 8; Table 2). Two- and 3-dimensional images were respectively obtained after processing with ImageJ and Imaris software (scale bar, 20 μm). Surface covered by platelets and thrombus volume were quantified by using ImageJ software (mean ± SEM). *P < .05, **P < .01, ***P < .001 vs healthy controls according to 2-way ANOVA and 1-sample t test.