Methylation analysis of GATA2 promoters. (A) Differential methylation analysis of putative promoters of the 2 expressed GATA2 isoforms using ERRBS (Prom-S and Prom-L). The following groups were compared: CEBPA_DM (n = 10), Control_ASE (n = 20), and Control_BE (n = 5). The y-axis indicates the percentage of methylation, averaged for all the CpG positions in each promoter region. (B) Differential methylation analysis of the promoters of the 2 expressed GATA2 isoforms using bisulfite treatment followed by amplicon sequencing. Note that the amplified regions (denoted as S and L) are selections of the sequences examined in the ERRBS data. Groups were defined as described: CEBPA_DM (n = 9), Control_ASE (n = 7), and Control_BE (n = 2). (C) Methylation changes in GATA2 promoters of paired diagnosis-remission samples from patients with CEBPA DM AML.