Gene expression in erythroblasts derived from WT and MT iPSCs. (A) Schema of the protocol for the erythroid differentiation from iPSCs and sample collection time points. Images of HPCs and OP9 cells were created using BioRender.com. (B) A heat map of the sample-to-sample distance and hierarchical clustering of iPSCs, CD34⁺ cells, CD43⁺CD34⁺CD38⁻Lin⁻ cells, and erythroblasts derived from 3 WT and 4 MT iPSC lines with RNA-seq. (C) Principal component analysis of erythroblasts derived from the 3 WT and 4 MT iPSC lines and bone marrow erythroblasts from a healthy donor and SA3. (D) Two GO analyses showing molecular function terms enriched for WT erythroblasts (top) and MT erythroblasts (bottom). Differentially expressed genes between WT erythroblasts and MT erythroblasts were used. (|fold change| >2; adjusted P < .05). (E) Gene set enrichment analysis of the heme metabolism data set showed an enrichment of WT erythroblasts. (F) A heat map of 13 genes characteristically expressed at the orthochromatic stage in the erythroblasts described in panel C. For WT1-erythroblasts and MT1-erythroblasts, the heat map shows the average expression levels of the 3 and 4 lines, respectively.