NUP98-fusion proteins dissociate from chromatin upon treatment with VTP50469 at a subset of critical genes. (A) Tornado plot and average signal plot depicting genome-wide NUP98-HOXD13^bio chromatin occupancy as determined by ChIPseq in mouse leukemia cells expressing NUP98-HOXD13^bio. (B) Gene tracks of MLL1, Menin, and NUP98-HOXD13^bio ChIPseq signal (rpm) at selected genes. (C) Violin plot depicting changes in NUP98-HOXD13^bio occupancy for previously defined NUP98-HOXD13 target genes¹³ that display >log2 fold change in gene expression (dark blue, n = 19 genes) vs all NUP98-HOXD13 targets (light blue, n = 295 genes) vs all genes (gray, n = 616 genes). (D) Scatterplot depicting all genes that display changes in both NUP98-HOXD13^bio (x-axis) and MLL1 (y-axis) chromatin occupancy as measured by ChIPseq in NUP98-HOXD13^bio mouse leukemia cells. Data are representative of 2 independent experiments. Two-way ANOVA with Dunnett’s multiple comparisons test was performed.