Titers and dominant apparent affinity constants of FVIII-specific IgG1 and IgA antibodies in hemophilia A patients stratified by HCV antibody status and in healthy donors. Presented are scatter dot blots for individual titers and dominant apparent affinity constants (K_As [M⁻¹]) for FVIII-specific IgG1 (A-B; titers: dark blue; affinities: light blue) and FVIII-specific IgA (C-D; titers: dark green; affinities: light green) in patients with (A,C) or without (B,D) anti-HCV antibodies and in healthy donors (B,D). Bold horizontal lines indicate the medians of titers and dominant K_As. Vertical bars indicate corresponding IQRs. P values for group comparisons by Mann-Whitney U tests are indicated. Statistically significant P values are highlighted in bold: p= 0.036 and marked with an asterisk: *P ≤ .050.